Low-Level MYKOVAM® Inoculation Enhances Growth Performance and Biomass Accumulation in Coffea arabica Seedlings
Keywords:
Arbuscular mycorrhizal, Biofertilizer, Leaf count, Parameters, Root and Shoot Biomass, Soil physico-chemical analysisAbstract
Coffee arabica is highly valued in the Philippines for its superior taste and increasing demand. However, the industry continues to face challenges such as low yield, poor bean quality, and susceptibility to pests and diseases. Successful seedling propagation requires the use of biofertilizers to enhance nutrient and water uptake, improve tolerance, and protect against root pathogens. This study evaluated the effects of varying levels of MYKOVAM® on the growth performance and biomass production of C. arabica seedlings under nursery conditions. A Randomized Complete Block Design (RCBD) was employed with five treatments and four replications: C (0 g MYKOVAM®), T1 (5 g), T2 (10 g), T3 (15 g), and T4 (20 g). A total of 300 one-month-old seedlings were inoculated at the recommended rates. Growth parameters measured after three months included percent survival, leaf number, root collar diameter, height gain, root length, root biomass, and shoot biomass. Results showed that T1 significantly improved survival, height gain, leaf count, root biomass, and shoot biomass compared to other treatments, though no significant difference was observed in root collar diameter. T2 produced the longest root length, while T3 and T4 recorded the lowest means across most parameters. These findings suggest that the application of 5 g MYKOVAM® is the most effective level for enhancing growth and biomass production of C. arabica seedlings, offering a sustainable approach to improving coffee cultivation in the Philippines.
References
Abadayan, J. G., and Coracero, E. E. (2024). Seedling Growth and Survival of the Endangered Pterocarpus indicus Willd. (Fabaceae) as Affected by Provenances and Biofertilizer Amendments. Forestist, 74(2).
Aggangan, N., Albano, A., Kasahara, E., and Ragragio, E. (2013). Survival, growth and Cu accumulation by non-mycorrhizal and mycorrhizal Jatropha curcas L. seedlings or cuttings in a grassland and in mine tailing soils. Journal of Environmental Science and Management, 16(2).
Agangan, N. S., Calayag, M. L., Salvan, A. J. and Velandres, J. C. (2011). Effect of Mykovam, Bio-N and inorganic fertilizers on growth, yield and nutrient status of sweet Sorghum (Sorghum bicolor) (L) Moench). Transaction of the National Academy of Science and Technology. Vol. 33, No. 1:103
Aggangan, N. S. and Morong, L. J. M. (2019): Influence of microbial inoculation on heavy metals absorption of three reforestation species. Philippine Journal of Crop Science 44: 18-27. https://ovcre.uplb.edu.ph/ journals
Aguilar, E. A., Elleva, L. I. F., Fabro, D. M. A., Garcia, G. R., Divina, F. I. and Aggangan, N. (2018). Arbuscular Mycorrhizal fungi increased root mycorrhizal association and enhanced seedling growth of abaca, papaya, and sugarcane.
Araujo, W.L., Dias, P.C., Moraes, G.A., Celin, E.F., Cunha, R.L., Barros, R.S. and DaMatta, F.M. (2008). Limitations to photosynthesis in coffee leaves from different canopy positions. Plant Physiol. Biochem. 46, 884–890.
Bad-e, R., Khorasani,R. and Tavakkoli, F. (2020). Determining seedling survival under nursery conditions. Journal of Horticultural Science and Technology, 13(1), 45_52.
Baltazar, M. D. and Fabella, J. M. A. O. (2020). Assessment of the genetic diversity of Philippine Arabica coffee (Coffea arabica L.) using SSR markers. Philipp J Sci, 149, 993 1003.
Bautista, J. O. P. and Buhong, N. D. (2024). Influence of Different Soil Amendments on the Growth Performance of Lipote (Syzygium polycephaloides (CB Rob.) Merr) Seedlings. Ecology, Environment & Conservation (0971765X), 30(4).
Bianchi, E., Bugmann, H., and Bigler, C. (2019). Early emergence increases survival of tree seedlings in Central European temperate forests despite severe late frost. Ecology and evolution, 9(14), 8238-8252.
Browning, D. (2018). How many coffee farms are there in the world. In ASIC conference, Portland, USA.
Castañeto, Y. T. (2001). Growth response of molave (Vitex parviflora Juss.) seedlings to Biocore and Mykovam. Philippine Journal of Science, 130(2), 97–101.
Davis, A. P., Gole, T. W., Baena, S. and Moat, J. (2012). The impact of climate change on indigenous arabica coffee (Coffea arabica): predicting future trends and identifying priorities. PloS one, 7(11), e47981.
del Rio, S. P., Aggangan, N. S. and Protacio, C. M. (2024). Alleviation of Soil Acidity Stress In Robusta Coffee By Arbuscular Mycorrhizal Fungi-Containing Biofertilizers. The Philippine Journal of Crop Science, 49(1), 49-55.
Dierauf, T. A. and Garner, J. W. (1996). Effect of initial root collar diameter on survival and growth of yellow-poplar seedlings over 17 years. Tree Planters Notes, 47, 30 33.
Elleva, L. I. F., Garcia, G. R., Divina, F. I., Fabro, D. M. A., Aguilar, E. A. and Aggangan, N. S. (2018). Response of MYKOVAM® treated lakatan banana plantlets to water deficit. Philippine Journal of Crop Science, 432, 56-62
Food and Agriculture Organization of the United Nations. (2021). Assessment of soil degradation in the Philippines: Impacts on agricultural productivity. FAO. https://www.fao.org/philippines/resources/ soil-degradation-report
Gonzales, J. Q. P., Lapuz, G. J. D., Reyes, A. M. D., Manabat, P. J. P., Garrido, J. E. M., Villaruel, H. V. and Angeles, M. R. (2023). Coffee leaf rust disease detection with deep learning algorithm and wireless sensor network integration. In TENCON 2023- 2023 IEEE Region 10 Conference (TENCON) (pp. 1052 1057). IEEE.
Jimoh, M. O. and Afolayan, A. J. (2020). Heavy metal up¬take and growth characteristics of Amaranthus caudatus L. under five different soils in a controlled environment. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(1), 417-425.
Lavelah, O. M., Edaño, M. L. S., Cruz, P. C. S. and Dizon, J. T. (2021). Growth, Yield and Grain Quality of Three Maize Varieties and Mungbean under Different Cropping Systems Inoculated with Mykovam®. International Journal of Research and Innovation in Social Science, 5(4), 330-337.
Markesteijn, L., & Poorter, L. (2009). Seedling root morphology and biomass allocation of 62 tropical tree species in relation to drought-and shade-tolerance. Journal of Ecology, 97(2), 311-325.
Magaña Arteaga, R., González Fuentes, J. A., López Cervantes, R. and Bañuelos Herrera, L. (2015). Efectode ácidos húmicos y fúlvicos en el crecimiento de lechuga (Lactuca sativa L.) bajo un sistema raíz flotante.
Miransari, M. (2013). Arbuscular mycorrhizal fungi and uptake of nutrients. In C. Varma, R. Prasad, & N. Tuteja (Eds.), Symbiotic Endophytes (pp. 253–270). Springer. https://doi.org/10.1007/978-3-642 39317- 4_13
Orchard, S. E., Stringer, L. C. and Manyatsi, A. M. (2017). Farmer perceptions and responses to soil degradation in Swaziland. Land Degradation & Development, 28(1), 46-56.
Shukla, S., Didwania, N. and Choudhary, R. (2025). Arbuscular mycorrhizal fungi: A pathway to sustainable soil health, carbon sequestration, and greenhouse gas mitigation. Springer. https://doi. org/10.1007/978-981-99-XXXX-X
Silvera-Pablo, C. C., Julca Otiniano, A., Herrera Toscano, L., Rivera-Ashqui, T. A., and Silva Paz, R. J. (2025). Assessment of Biofertilizers and Humic Acids on the Growth of Coffee Varieties in Nursery: Experimental Study in Chanchamayo, Peru. Int J Agri Biosci, 14(1), 145-152.
Sollestre, H. R. G., & Hao, K. J. M. (2026). Assessing the Effects of Different Application Rate of Mykovam on the Growth and Yield Performance of Tomato. International Journal of Research and Innovation in Social Science (IJRISS), 10(4).
Wintgens, J. N. (Ed.). (2012). Coffee-growing, processing, sustainable production: A Guidebook for Growers, Processors, Traders and researchers. John Wiley & Sons.
World’s Top Exports. (2026). Coffee exports by country: Global rankings 2026. World’s Top Exports. https://www.worldstopexports.com/coffee-ex¬ports-country/
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Copyright (c) 2026 Krissa May Trucilla, Merielyn M. Boce, Marienella P. Aquino, Mark Jerick S. Memita, Wandeg C. Diguinat, Ashlie A. Tagle, Mark Christian T. Facun, Novelyn D. Buhong, Jan Orville P. Bautista

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